Coding

Part:BBa_K4595020:Design

Designed by: Tang rui   Group: iGEM23_HUST-China   (2023-10-11)

_NOTOC__ gshB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
    Illegal BamHI site found at 543
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 520
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon-optimized its sequence.


Source

Synechocystis PCC6803

References

[1]Lu, S. C. (2013). GLUTATHIONE SYNTHESIS. Biochemica et Biophysica Acta, 1830(5), 3143–3153. http://doi.org/10.1016/j.bbagen.2012.09.008 [2]Badri H, Monsieurs P, Coninx I, Wattiez R, Leys N. Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005. Microbiologyopen. 2015 Apr;4(2):187-207. doi: 10.1002/mbo3.229. Epub 2015 Feb 12. PMID: 25678338; PMCID: PMC4398503. [3]Narainsamy K, Farci S, Braun E, Junot C, Cassier-Chauvat C, Chauvat F. Oxidative-stress detoxification and signalling in cyanobacteria: the crucial glutathione synthesis pathway supports the production of ergothioneine and ophthalmate. Mol Microbiol. 2016 Apr;100(1):15-24. doi: 10.1111/mmi.13296. Epub 2016 Feb 9. PMID: 26713511.